Genomes 5 has been completely revised and updated. It is a thoroughly modern textbook about genomes and how they are invesgated. As with previous Genomes edions, techniques come first, then genome anatomies, followed by genome funcon, and finally genome evoluon. The genomes of all types of organism are covered: viruses, bacteria, fungi, plants, and animals, including humans and other hominids. Genome sequencing and assembly methods have been thoroughly revised to include new developments in longread DNA sequencing. Coverage of genome annotaon emphasizes genome-wide RNA mapping, with CRISPR-Cas 9 and GWAS methods of determining gene funcon covered. The knowledge gained from these techniques forms the basis of the chapters that describe the three main types of genomes: eukaryoc, prokaryoc (including eukaryoc organelles), and viral (including mobile genec elements). Coverage of genome expression and replicaon is truly genomic, concentrang on the genome-wide implicaons of DNA packaging, epigenome modificaons, DNAbinding proteins, non-coding RNAs, regulatory genome sequences, and protein-protein interacons. Also included are examples of the applicaons of metabolomics and systems biology. The final chapter is on genome evoluon, including the evoluon of the epigenome, using genomics to study human evoluon, and using populaon genomics to advance plant breeding. Established methods of molecular biology are included if they are sll relevant today and there is always an explanaon as to why the method is sll important. Genomes 5 is the ideal text for upper-level courses focused on genomes and genomics