TY  - BOOK
AU  - edited by Luo, Yonglun
TI  - CRISPR gene editing: methods and protocols 
SN  - 9781493991693
U1  - 660.65 LUO-Y 
PY  - 2019///
CY  - New York
PB  - Humana Press
KW  - CRISPR-associated protein 9
KW  - Gene editing
KW  - Genetic engineering
KW  - Human genetics
N1  - This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com
ER  -