TY  - BOOK
AU  - Snyder, Richard O.
TI  - Adeno-associated virus: methods and protocols edited by
SN  - 9781493961757
U1  - 576.85 SNY-R 
PY  - 2011///
CY  - New York
PB  - Springer
KW  - Gene therapy--Laboratory manuals.
KW  - Genetic transformation--Laboratory manuals.
KW  - Parvoviruses--Laboratory manuals.
KW  - Genetic vectors
N1  - Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of  gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding ― or at least an appreciation of ― the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field
ER  -