TY - BOOK AU - Capes-Davis,Amanda AU - Capes-Davis,Amanda AU - Freshney,R.Ian TI - Freshney's culture of animal cells: a manual of basic technique and specialized applications SN - 9781119513018 AV - QH585.2 .F74 2021 U1 - 571.538 CAP-A 23 PY - 2021/// CY - UK PB - Wiley Blackwell KW - Tissue culture KW - Laboratory manuals KW - Cell culture N1 - Revised edition of: Culture of animal cells : a manual of basic technique and specialized applications by R. Ian Freshney. Seventh edition. [2016]; Includes bibliographical references and index N2 - "Cell culture is the process used to keep cells alive in the laboratory. The process has undergone many changes since its discovery in 1907, as better techniques, equipment, and reagents continue to be developed. Cell culture now relies on a toolkit of techniques (e.g. aseptic technique), equipment (e.g. biosafety cabinet), and reagents (e.g. flasks and growth medium). Cells can be maintained and grown from many species and tissues, ranging from humans to fish, and from skin to brain. New methods and uses are emerging, allowing scientists to grow stem cells and develop more effective models through three-dimensional culture. However, cell culture can be challenging. Problems can affect any cell culture practitioner, even the most seasoned expert. Training is needed to ensure aseptic conditions, skilled handling, and an awareness of safety issues and of the purity and identity of the material being handled"-- ER -