The laboratory performs a wide variety of different biochemical tests in whole blood, serum/plasma and other bio fluids by the use of a wide range of equipment, from the simple most basic to automated, modern and sophisticated systems. Clinical biochemistry is that branch of laboratory medicine in which chemical and biochemical methods are applied to the study of disease while in theory this embraces all non-morphological studies, in practice it is usually, though not exclusively, confined to studies on blood and urine because of the relative ease in obtaining such specimens although analysis are made on other body fluids such as gastric aspirate and cerebrospinal fluid. A major contribution to bacterial was the development of methods using solid medium for the cultivation of bacteria. Koch was convinced that microbes caused some diseases. However, to test this idea, he needed to isolate the causative agent. Almost all samples from diseased animals or any natural surface contained many different microbes and it was impossible to tell which one was the problem. A method was needed to separate these different bacteria. Enzymes bind temporarily to one or more of the reactants of the reaction they catalyze. In doing so, they lower the amount of activation energy needed and thus speed up the reaction. Enzymes are biomolecules that catalyze chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules at the beginning of the process are called substrates, and the enzyme converts them into different molecules, the products. the book provides readers with a logical framework for conducting modern biochemical, biotechnology and molecular biology research.