000			02052nam a22001937a 4500
008			240328b2011 |||||||| |||| 00| 0 eng d
020			_a9781493961757
082			_a576.85 SNY-R
100			_aSnyder, Richard O.
245			_aAdeno-associated virus : _bmethods and protocols edited by _cRichard O. Snyder and Philippe Moullier
260			_aNew York _bSpringer _c2011
300			_a462p.
440			_aMethods in molecular biology
500			_aToday, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding ― or at least an appreciation of ― the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.
650			_aGene therapy--Laboratory manuals.
650			_aGenetic transformation--Laboratory manuals.
650			_aParvoviruses--Laboratory manuals.
650			_aGenetic vectors.
999			_c92215 _d92215